Soil microbial community responding to moderately elevated nitrogen deposition in a Japanese cool temperate forest surrounded by fertilized grasslands

Nagano, Hirohiko; Nakayama, Masataka*; Katata, Genki*; Fukushima, Keitaro*; Yamaguchi, Takashi*; Watanabe, Makoto*; Kondo, Toshiaki*; Atarashi-Andoh, Mariko; Kubota, Tomohiro*; Tateno, Ryunosuke*; et al.

Soil Science and Plant Nutrition, 67(5), p.606 - 616, 2021/10

https://doi.org/10.1080/00380768.2021.1974799

We analyzed the relationships between nitrogen deposition (deposition of nitrate and ammonium ions) and soil microbial properties in a cool temperate forest surrounded by normally fertilized pasture grasslands in northern Japan. The aim of the present study was to gain the primary information on soil microbial response to moderately elevated nitrogen deposition ( 10 kg N ha y). We established three experimental plots in the forest edge adjacent to grasslands and other three plots in the forest interior at least 700 m away from the grasslands. During May to November 2018, nitrogen deposition in each plot was measured. In August 2018, litter and soil (0-5 cm depth) samples were collected from all plots to measure net nitrogen mineralization and nitrification rates as indicators of microbial activity, and microbial biomass carbon and nitrogen and various gene abundances (i.e. bacterial 16S rRNA, fungal ITS, bacterial amoA, and archaeal amoA genes) as indicators of microbial abundance and structure. Nitrogen deposition in the forest edge was 1.4-fold greater than that in the forest interior, even while the maximum deposition was 3.7 kg N ha. Nitrogen deposition was significantly correlated to the net nitrogen mineralization and nitrification rates and the 16S rRNA and bacterial amoA gene abundances. Microbial community structures were different between litter and soil samples but were similar between the forest edge and interior. Significant correlations of nitrogen deposition to the soil carbon to nitrogen ratio, and the nitrate and ammonium contents were also observed. Thus, our results show that moderately elevated nitrogen deposition in nitrogen-limited forest edges can stimulate microbial activities and abundances in soils.

Laboratory examination of greenhouse gaseous and microbial dynamics during thawing of frozen soil core collected from a black spruce forest in Interior Alaska

Nagano, Hirohiko; Kim, Y.*; Lee, B.-Y.*; Shigeta, Haruka*; Inubushi, Kazuyuki*

Soil Science and Plant Nutrition, 64(6), p.793 - 802, 2018/12

https://doi.org/10.1080/00380768.2018.1525267

In this study, we conducted an incubation experiment on a frozen soil core collected from a black spruce forest in Interior Alaska, in order to investigate potential changes in greenhouse gaseous (GHG) and microbial dynamics during thawing of frozen soil. The soil thawing is an important environmental process determining the annual GHG balance in the northern high-latitude ecosystem. A core spanning the ground surface to upper permafrost with a depth of 90 cm was vertically grouped into three layers (top, middle, and bottom layers). Then, 12 soil samples from 3 layers (i.e., 4 soil samples per layer) were incubated for 3 weeks, and net carbon dioxide (Co) and methane (CH) release/uptake rates were estimated. During the incubation, temperature was changed weekly from 0 to 5, then 10C. The net amounts of CO released by six of the eight soil samples from the top and middle layers were 1.5 to 19.2-fold greater at 5C than at 0C, while the release at 10C was reduced in the cases of three of these six soil samples. Net CH release was the greatest in bottom-layer soil samples incubated at 0C. Then, low but apparent CH release was observed in top and middle-layer soil samples incubated at 0C. At 5 and 10C, net CH release from bottom-layer soil samples was decreased. Then, net CH uptake was observed in the top and the middle-layer soil samples. Both net uptake and release of CH were reduced upon the addition of a chemical inhibitor (i.e., 2-bromoethane sulfonate) of anaerobic methanotrophic and methanogenic activity. The bacterial and archaeal community structures based on 16S rRNA amplicon analysis were changed along the depth, while they were less changed during thawing. Thus, it was found that soil GHG dynamics responded sensitively and variously to thawing, while there was less change in 16S rRNA-based microbial community structures during the thawing prog

A Quick incorporation of N into the soluble high-molecular compound in rice ( L.) roots by application of N-labeled nitrate/nitrite

Yoneyama, Tadakatsu*; Suzui, Nobuo; Ishioka, Noriko; Fujimaki, Shu

Soil Science and Plant Nutrition, 57(2), p.279 - 282, 2011/05

https://doi.org/10.1080/00380768.2011.576399

Factors controlling potentially mineralizable and recalcitrant soil organic matter in humid Asia

Kadono, Atsunobu; Funakawa, Shinya*; Kosaki, Takashi*

Soil Science and Plant Nutrition, 55(2), p.243 - 251, 2009/04

https://doi.org/10.1111/j.1747-0765.2008.00355.x

To understand the dynamics of soil organic matter (SOM) in humid Asia, several properties were tested to estimate the amounts of potentially mineralizable carbon (PMC) and nitrogen (PMN) as well as recalcitrant carbon (ROC) and nitrogen (RON). Eighty-nine surface soil samples were collected in Thailand, Indonesia and Japan from cropland and forest ecosystems. The fresh soils were incubated under constant conditions, and the CO and the mineral N from the soils were monitored. PMC and PMN were determined by fitting models to the cumulative curves of the emitted C and N. ROC and RON were determined by the subtraction of PMC and PMN from TC and TN, respectively. Using multiple regression analysis with the stepwise method, PMC and PMN was well estimated by carbon and nitrogen content of light fraction (LFC and LFN) and clay content, while ROC and RON were estimated by LF-OM, amorphous materials and clay content, suggesting the importance of parent materials in this region.

Mn translocation in barley monitored using a positron-emitting tracer imaging system

Tsukamoto, Takashi*; Nakanishi, Hiromi*; Kiyomiya, Shoichiro*; Watanabe, Satoshi; Matsuhashi, Shimpei; Nishizawa, Naoko*; Mori, Satoshi*

Soil Science and Plant Nutrition, 52(6), p.717 - 725, 2006/12

https://doi.org/10.1111/j.1747-0765.2006.00096.x

Visualization of O-water flow in tomato and rice in the light and dark using a positron-emitting tracer imaging system (PETIS)

Mori, Satoshi*; Kiyomiya, Shoichiro*; Nakanishi, Hiromi*; Ishioka, Noriko; Watanabe, Satoshi; Osa, Akihiko; Matsuhashi, Shimpei; Hashimoto, Shoji; Sekine, Toshiaki; Uchida, Hiroshi*; et al.

Soil Science and Plant Nutrition, 46(4), p.975 - 979, 2000/12

https://doi.org/10.1080/00380768.2000.10409163

no abstracts in English

TTC reduction by submerged soils

*; *;

Soil Science and Plant Nutrition, 29(4), p.489 - 497, 1983/00

https://doi.org/10.1080/00380768.1983.10434652

no abstracts in English

Further Study on Iodine Toxicity in Relation to 「Reclamation Akagare」 Disease of Lowland Rice

; *

Soil Science and Plant Nutrition, 16(5), p.192 - 194, 1971/00

https://doi.org/10.1080/00380768.1970.10432839

no abstracts in English

Absorption of radioactive strontium by soil, especially in relation to nature cesium

; K.Yeh*; S.Mitsui*

Soil Science and Plant Nutrition, 1, p.152 - 156, 1961/00

no abstracts in English